In this work we investigated whether streptamer technology could purify WT1-specific CD8+ T cells, what is important for the development of adoptive immunotherapy. Sample from HLA/A2+ HDs were identified and selected by streptamer. The function of selected CD8+ T cells was identified by the staining of phenotypic markers. The results showed that streptamer permits the detection and selection of WT1-specific CD8+ T cells in the PBMCs from HDs. The naïve function of selected CD8+ T cells was preserved and most selected CD8+ T cells demonstrated an effector T cell immunophenotype.
