A systems biology approach is used to investigate a key regulator of energy sensing, the AMPK/ Snf1 pathway. Therefore, a stimulus is derived considering transmembrane glucose transport. Fluorescent microscopy data of Mig1, a Snf1 pathway component, were processed through a mathematical approach. The data were compared to simulations of alternative network structures suggested to underlie the Snf1 pathway. Certain structures could be excluded. Besides, applying quantitative measures showed that the design of a MAPK pathway is favourable for the transmission of fast signals with short duration.
