The aim of this work is to analyze the effects of temperature on dielectric parameters of Human Red Blood Cells (HRBCs). The cells were suspended in external media with different conductivities and observed at different temperatures. An AC electric field was applied. The cell parameters were obtained by fitting the experimental points with the model. Effects on cell volume and cell radius led to a change in the interior ionic concentration. Cytoplasmic conductivity was calculated using the Debye-Hückel-Onsager relation. An exponential relationship of the conductivity to temperature was found.