HumanNPCs (hNPCs) are the suitable cell source in translation medicine for neurodegenerative disease. In the present study, effects of Wnt3a on the differentiation of hNPC were studied. The results showed that Wnt3a induces canonical and non canonical Wnt pathways. Furthermore, Wnt3a increases Ca2+ target genes CaMKII and Pyk2 in the differentiating VM cells. The results demonstrated that Pyk2 decreases GSK3b activity and stabilizes b-catenin, suggesting there is an intercross between Wnt/b-catenin and Wnt/Ca2+-pathway via Pyk2.<eng>
