Publikation: Dissertationsschrift

Biochemical analysis of the -terpineol/1,8-cineole cyclization reaction of the cineole synthase of Nicotiana suaveolens

Grunddaten
Abstract
Autoren
Einrichtung

Grunddaten

Titel	Biochemical analysis of the -terpineol/1,8-cineole cyclization reaction of the cineole synthase of Nicotiana suaveolens
Erscheinungsjahr	2015
Publikationsform	Elektronische Ressource
Publikationsart	Dissertationsschrift
Sprache	Englisch
Letzte Änderung	30.03.2022 13:01:58
Bearbeitungsstatus	durch UB Rostock abschließend validiert
Dauerhafte URL	http://purl.uni-rostock.de/fodb/pub/49952
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Abstract

19 mutants of the cineole synthase of N. suaveolens were generated by site-directed mutagenesis in order to substitute the amino acids of the cineole synthase with amino acids of the equivalent position of the terpineol synthase of N. langsdorfii. The mutant enzyme F266S carrying a phenylalanine replacement by serine showed an inverted product spectrum compared to the wild type enzyme. Terpineol was the major compound in the product profile of the mutant F266S. It is hypothesized that the synthesis of cineole proceeds independent of terpineol and via -terpinyl hydronium ion (Wise et al. 2002).

Autor

Brosemann, Anne

Einrichtung

MNF/Institut für Biowissenschaften (IfBI)