Publikation: Dissertationsschrift

Electrochemical hybridization assay for DNA and asymmetric PCR products detection by labeling with osmium tetroxide

Grunddaten
Link
Abstract
Autoren
Einrichtung

Grunddaten

Titel	Electrochemical hybridization assay for DNA and asymmetric PCR products detection by labeling with osmium tetroxide
Erscheinungsjahr	2016
Verlag	Universität Rostock
Verlagsort	Rostock
Publikationsform	Elektronische Ressource
Publikationsart	Dissertationsschrift
Sprache	Englisch
Letzte Änderung	13.04.2018 09:30:40
Bearbeitungsstatus	durch UB Rostock abschließend validiert
Dauerhafte URL	http://purl.uni-rostock.de/fodb/pub/56103
Links zu Katalogen

Abstract

This dissertation describes the hybridization assay on gold electrodes for electrochemical detection of DNA and polymerase chain reaction (PCR) products by means of the electrochemically active compound osmium tetroxide bipyridine. Single base mismatches can be discriminated for their different thermal behavior. We further optimized protective-strand design, surface-probe density, and hybridization temperature. Besides, we also investigated to obtain labeled single stranded DNA targets from asymmetric PCR after labeling forward primer with osmium tetroxide for electrochemical detection.

Autor

Ni, Xiaoqi

Externer Link

Beschreibung	Link
Link zur Online-Ressource (kostenfrei zugänglich ohne Registrierung)	http://rosdok.uni-rostock.de/resolve/urn/urn:nbn:de:gbv:28-diss2018-0038-9

Einrichtung

MNF/Institut für Chemie (IfCh)