Publikation: Dissertationsschrift

Investigating the mechanism of a novel glycine-dependent riboswitch and a putative non-coding regulatory RNA in Streptococcus pyogenes

Grunddaten
Abstract
Autoren
Einrichtungen

Grunddaten

Titel	Investigating the mechanism of a novel glycine-dependent riboswitch and a putative non-coding regulatory RNA in Streptococcus pyogenes
Erscheinungsjahr	2017
Verlag	Universität Rostock
Verlagsort	Rostock
Publikationsform	Elektronische Ressource
Publikationsart	Dissertationsschrift
Sprache	Englisch
Letzte Änderung	30.06.2018 05:03:35
Bearbeitungsstatus	durch UB Rostock abschließend validiert
Dauerhafte URL	http://purl.uni-rostock.de/fodb/pub/57015
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Abstract

We investigated gene expression regulation by a putative glycine(Gly) riboswitch located in the 5-UTR of a SAF protein gene in the S.pyogenes. Gly-dependency was studied using a luciferase reporter gene system. Maximal reporter gene expression happened in the presence of low Gly concentrations. RT-qPCR showed that in the presence of Gly (1 mM), expression of the gene was downregulated. Growth in the presence of 0.1 mM Gly led to the production of a full-length transcript. We conclude that the Gly riboswitch in S. pyogenes represses gene expression in the presence of high Gly concentrations.

Autor

Khani, Afsaneh

Einrichtungen

MNF/Institut für Biowissenschaften (IfBI)

UMR/Institut für Medizinische Mikrobiologie, Virologie und Hygiene