Publikation: Dissertationsschrift

Development and application of affinity-mass spectrometry to identify protein-carbohydrate interactions

Grunddaten
Abstract
Autoren
Einrichtung

Grunddaten

Titel	Development and application of affinity-mass spectrometry to identify protein-carbohydrate interactions
Erscheinungsjahr	2022
Verlag	Universität Rostock
Verlagsort	Rostock
Publikationsform	Druckschrift
Publikationsart	Dissertationsschrift
Sprache	Englisch
Letzte Änderung	11.02.2023 06:01:55
Bearbeitungsstatus	durch UB Rostock abschließend validiert
Dauerhafte URL	http://purl.uni-rostock.de/fodb/pub/69295
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Abstract

Affinity mass spectrometry using selective and pressure-enhanced proteolytic excision and extraction combined with MALDI and ESI-MS has been applied to the identification of epitope binding sites of GalNac and blood group oligosaccharides in lectins. The epitope peptides identified comprise all essential amino acids involved in carbohydrate recognition, in complete agreement with available X-ray structures. Coupling of a specific peptide glycoprobe to the gold surface of a biosensor chip was successfully employed for identification of carbohydrate epitopes and affinity measurements.

Autor

Baschung, Yannick

Einrichtung

UMR/Institut für Immunologie